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At concentrations as low as 0.11 ng/mL, endotoxins can activate innate immune pathways in mammalian cells including NF-B and inflammasome signaling which directly confound any study involving cytokine release, cell viability, differentiation, or proliferation

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Science 375:12541261 Song MO, Li J, Freedman JH (2009) Physiological and toxicological transcriptome changes in HepG2 cells exposed to copper