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-glutamyl kinase (GK) encoded by PRO1 in eukaryotes or proB in prokaryotes, the first enzyme in proline biosynthetic pathway, catalyzes the formation of intermediate -glutamyl phosphate, which if accumulated could partially react with cysteine to generate -GC, and then Gsh2 adds glycine to -GC to form GSH
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Given these results, it is important to consider the time point of therapeutic administration, as earlier intervention may yield greater treatment efficacy, as evidenced by the age-dependent success in the mouse studies
The primers used were 5-caccatcacctcctggaaga-3 and 5-gggtgcagaatctcgagttg-3 for Pck1 , 5-gtggcagtggtcggagact-3 and 5-acgggcgttgtccaaac-3 for G6pc , 5-tacagagtgctggccaagag-3 and 5-ttcaaggatgcactggagag-3 for Cyp7a1 , 5-tagccctctttcctccactcata-3 and 5-gaagcgatcgaacctaaattcct-3 for Cyp7b1 , 5-gcctcacctatgggatcttca-3v and 5v-tcaaagcctgacgcagatg-3v for Cyp27a1 , 5-ggctggcttcctgagcttatt-3 and 5-acttcctgaacagctcatcgg-3 for Cyp8b1 , 5-acctgtctaacctcttcacc-3 and 5-caatgctgaggttcatgtcc-3 for Slc10a1 , 5-ggaagattgttggcccgatt-3 and 5-agtgggagttatggtcaggt-3v for Slco1a1 , 5-gcgtcgtgattagcgatga-3 and 5-atggcctcccatctcctt-3 for Hprt
Examples include grilled salmon with a walnut-arugula salad and lentil stew with spinach and tomato
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