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They can be categorized into synthetic hydrogels, polysaccharide-based hydrogels, and peptide (protein)-based hydrogels

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Blots were rinsed thrice (0.1% Tween20 in Tris-buffered saline, 5 min each), followed by incubation with peroxidase-conjugated secondary antibody (Dako) for 2 h at room temperature

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