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The reinforcing effect and DA level induced by DNC + NIC were greater than the sum of the two parts, suggesting that the relationship of the DNC group to nicotine was synergistic rather than additive, and the synergistic effect was further supported by the unique enhancement of the DNC + NIC group in locomotor activity compared to the NIC group (Figure 2C) and anxiety performance (Figure 2D)

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We conducted a sensitivity analysis within current vapers comparing trends among daily versus non-daily users (ie, testing the interaction between frequency of vaping [daily or non-daily] and time)