However, sourcing, dosage, and administration are everything
If binding of one IGF1 molecule to the IGF1R dimer is sufficient for receptor activation, then both the WT:WT and WT:F701A dimers are expected to be active, and the IGF1R activity in cells transfected with WT and F701A plasmids will be 75% of that in cells transfected with the WT plasmid alone
The complexity increases but remains manageable with proper planning
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Ren H, Wu ZTJ, Tao H, Zou W,Cao Z, Wen B,Cai ZDJ, Deng Z
