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Media and culture conditions Wild-type yeast strains were pre-cultured in 5 mL of YP20D medium containing 10 g/L yeast extract, 20 g/L Bacto Peptone, and 20 g/L glucose at 30 C and 250 rpm for 48 h

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In the in vivo study, the proliferative indices of adenovirus AdH1-siRNA/Met-injected mouse tumors were lower (23.4%) than the adenovirus AdH1-null injected tumors (69.8%) and mock-injected tumors (72.8%)

1, reaction 7) and an acceleration of the alternative transmethylation (Fig