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Validated in a variety of sample types Fast, accurate assay results in less than 30 minutes Convenient 96 well plate format for higher throughput

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In some embodiments, a formulation or composition disclosed herein is applied on the tympanic membrane

This limitation is acknowledged, and future iterations of the framework may incorporate histology-stratified analyses as more structured and mechanistically detailed data become available

2.6 Western blotting (WB), real-time quantitative PCR (RT-qPCR) and reactive oxygen species (ROS) analysis WB analysis, RT-qPCR, and ROS analysis were performed as previously described (Pang et al., 2022)