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The column was washed with 20 bed volumes of equilibration buffer to remove any non-specific bound proteins

It is likely that the larger number of statistically significant changes to the microbiome observed in the groups switched from WD to LD reflects their more numerous exposures to different diets, longitudinal changes in dietary exposure, the stress associated with diet switch, the negative energy state achieved by treatment and/or diet-driven weight loss, or a combination of these factors, but it may also be related to differences in terminal body weight and fat mass since both adiposity and diet composition have been shown to modulate the mouse gut microbiome profile

Within these research contexts the blend has been used as a tool to probe somatotropic-axis signaling, lipolysis and visceral-adipose models, and recovery and body-composition endpoints in non-clinical settings

Understanding the roles of dendritic cells in MASH requires precise characterization of their subsets in experimental models and rigorous validation of these findings in human MASH