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Quantitation of serum nicotine, cotinine, adrenalin, and -endorphin via Ultra-fast liquid chromatography-tandem mass spectrometry (UFLC-MS/MS) Nicotine and cotinine This study used a validated SHIMADZU UFLC-MS/MS system (Waters Corporation, Milford, MA, USA) to determine the concentration of nicotine and cotinine in rat serum