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Cureus, [online] 17(1).

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Although exploratory experimental studies currently indicate that ferroptosis is involved in the pathology of DN and can be targeted and intervened pharmacologically, the detailed mechanism still needs to be elucidated

doi: 10.3390/nu15081831 148 El-DalatonyMMXJGRoEPPRoPLiMicrobiotaG

The hAMY 3 R cells were cultured in growth medium consisting of DMEM (Gibco, #31966-021) supplemented with 10% v/v FBS (Gibco, #16140-071), 1% v/v Penicillin-Streptomycin (Gibco, #15140-122), 0.5 mg/mL Geneticin G418 (Gibco, #10131-027), 0.4 mg/mL Hygromycin (Invitrogen, #10687010) and 250 nM Methotrexate (Sigma, A6770)