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The final cell suspensions were analyzed by flow cytometry to test the intensity of DCFH-DA fluorescence at excitation and emission wavelengths of 480 nm and 530 nm, respectively

De novo synthesis of GSH from cysteine and glutamic acid involves catalysis by glutamate-cysteine ligase (GCL) to form gamma-glutamyl cysteine (g-GC) and the subsequent addition of glycine to g-GC by glutathione synthase (GS) [2]

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If the body experiences glutathione depletion, it can lead to an increase in oxidative stress, which damages cells and contributes to the development of chronic diseases