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Extracted samples were vortexed at 4 C for 10 min, cleared by centrifugation (17,000 g , 10 min, 4 C) and then supernatants were analysed by LCMS

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Hence, cilofungin, an echinocandin B derivative with a 4-octyloxy benzoate side chain, was synthesized but was withdrawn from further studies due to poor water solubility and the toxicity of its co-solvent system containing 26% polyethylene glycol [160]

doi: 10.1016/j.bioactmat.2025.06.027 196 ZhangL.ZhangY.MaY.WangZ.WanZ.SongY.et al

e , Top, volcano plot showing relative fold change (log 2 ) in ER-metabolite abundance versus log 10 (P value) from ER-tag HEK293T cells treated with thapsigargin compared to untreated