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Deglutamylation activities using -glutamyl- p -nitroanilide as the substrate were determined spectrophotometrically according to a previously described method (Orlowski and Meister, 1963), with slight modifications, as follows: the reaction mixture, which consisted of 0.0125 g l -1 yeast crude protein, 50 mM Tris-HCl (pH 8.0), 10 mM glycylglycine, and 1 mM -glutamyl- p -nitroanilide, was incubated for 6 h at 37C, and p -nitroaniline released from -glutamyl- p -nitroanilide was monitored at 412 nm

A cross-sectional (1)H-magnetic resonance spectroscopy ((1)H-MRS) study

Administering 1 mg/kg/day of Donafenil confirmed its ability to inhibit the activation of human hepatic stellate cells, thereby exerting a multi-targeted antifibrotic effect [58]

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