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For colony formation assay, cells were cultured in 24-well tissue culture plates for a week to form colonies before being fixed with 10% neutral formalin, stained with 0.5% crystal violet solution, and having the dye extracted by adding 10% acetic acid

You can think of them as smaller and simpler versions of proteins, which are made of longer chains of amino acids

This timing allows the probiotics and botanicals to soothe and strengthen your gut lining before you start fueling, helping prevent bloating before it even starts