Targeted metabolomics data collection For each sample, 20 L CSF or 5 L plasma was extracted by brief sonication in 200 L or 300 L extraction buffer, respectively (80% methanol, 25 mM ammonium acetate, 2.5 mM sodium ascorbate in LCMSgrade water), supplemented with isotopically labeled amino acids (Cambridge Isotope Laboratories, MSK-A2-1.2, Tewksbury, MA USA), aminopterin, and reduced glutathione (CNLM-6245-10)
& Harding, I
doi: 10.1023/A:1022899115220 124 MilanskiMDegasperiGCoopeAMorariJDenisRCintraDEet al
doi:10.1097/icu.0b013e3283622718
A similar analysis found that the changes in BUN/Creatinine from beginning of phase 1 to beginning of phase 2 could be partially predicted (adjusted R 2 = 0.51, p = 0.05, with the most significant factors being 9 th dose Hg (p = 0.01), change in glutathione (p = 0.02), 9 th dose Tl (p = 0.04), and 9 th dose Pb (p = 0.05)