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Equal amounts of protein in sample loading buffer were separated by gel electrophoresis, and transferred onto nitrocellulose membranes [5]

Briefly, male C57bl/6 mice were sensitised on day 0 and 14 with either OVA (10 g/ mouse, i.p.) with Alum (diluted 1:1 with saline, 100 l i.p.) or HDM (0.5 g/kg in 100 ul, i.p

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