The primary antibodies employed were: P53 (Proteintech, 60283-2-Ig), pSer46-P53 (CST, 2521), FOXO4 (Proteintech, 21535-1-AP), P21 (CST, 2947), P16 (CST, 18769), BAX (CST, 2772), BCL2 (CST, 3498), Ki-67 (Proteintech, 84192-4-RR), Lamin B (CST, 13435), Cleaved Caspase 3 (CST, 9664), -H2AX (Abcam, ab81299), and GAPDH (Abcam, ab8245) as an internal reference for normalizing protein expression levels
Glutathione is actually a protein made up of 3 amino acids
Isoforms GPx1 and GPx4 probably have a protective role in the development of AMD [81,82]
However, rats treated with glycitin exhibited a reduction in their hippocampal dark neurons
This allows cells to quickly adapt to changing physiological conditions by altering the composition and function of gap junctions (Fallon and Goodenough 1981)