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As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day

10.1056/NEJMe020106

The following mechanisms are responsible for high vitamin B12 blood values in such diseases: Overproduction of haptocorrin Mistaken excretion of vitamin B12 Release of large quantities of B12 from the bodys store in the liver Production of B12 analogues through bacterial overgrowth Below is a list of the most common diseases associated with very high vitamin B12 blood levels