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Plates were read 5 min following drug addition using a PheraStar FSX equipped with a nanoBRET-compatible optic module (LUM 610 450) (BMG Labtech)

In arthritis, inflammation breaks down tissues in the joints, leading to pain and reduced mobility

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As seen in Supplemental Figure 4B, the sum of total intracellular hydroperoxy- and hydroxy-phospholipids following IL-13 stimulation was approximately 2 nmol/mg protein, that is, sufficient amounts to quantifiably impact the change in intracellular GSH (and the increases in GSSG) under these two conditions, as shown in Supplemental Figure 4C under similar conditions