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Patch assay Single colonies of strains W303-1b, W303-1b/gsh1, W303-1b/gsh1/pro1 and W303-1b/gsh1/proB were diluted in distilled H 2 O and patched (about 500 cells/L) on YPD plates containing various concentrations of H 2 O 2

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Solid-phase and solution-phase manufacturing

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By immunohistochemical staining and western blot analysis, an increase in MMP-9 expression in the CS group was observed compared to that in the control group, and pretreatment with GHK-Cu reduced MMP-9 expression compared to that in the CS group (Figures 2AC)