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Western blot Protein was extracted from renal tissues or MTEC cells, and concentrations were measured using a BCA protein assay kit

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However, Zn 2+ single ions treatment is not strong enough to stimulate hair regeneration in AGA, and one of our hypotheses is that the combination of Zn 2+ with Cu 2+ and Qu together may synergistically enhance hair regeneration in AGA, since previous studies demonstrated that the combination of Cu 2+ with amino acids could effectively activate the hair follicle stem cells in telogen phase and promote cell proliferation [76], and polyphenolic compounds (e.g., gallic acid and Qu) exhibit amino acid-like activity in some conditions [77,78], although it is unclear whether Cu 2+ , Qu alone, or their combination with Zn 2+ would have a regulatory effect on hair follicle stem cells in the telogen phase

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