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New horizons in tumor microenvironment biology: challenges and opportunities

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The cDNA was probed for the constitutive, intron-spanning YRA1 gene using the custom-designed primers 5-TGTCGGTGGTACTCGTGGTA-3 ( YRA1 -F) and 5-TAGTCCGCCATTTCCTTGTC-3 ( YRA1 -R), at an annealing temperature of 54 C, together with the Thermo Scientific 2X ReddyMix PCR Master Mix

Exploration and optimization of conditions for quantitative analysis of lignans in Schisandra chinensis by an online supercritical fluid extraction with supercritical fluid chromatography system