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In some embodiments, the otic formulation or composition comprises about 0.8% by weight of the povidone

Deglutamylation activities using -glutamyl- p -nitroanilide as the substrate were determined spectrophotometrically according to a previously described method (Orlowski and Meister, 1963), with slight modifications, as follows: the reaction mixture, which consisted of 0.0125 g l -1 yeast crude protein, 50 mM Tris-HCl (pH 8.0), 10 mM glycylglycine, and 1 mM -glutamyl- p -nitroanilide, was incubated for 6 h at 37C, and p -nitroaniline released from -glutamyl- p -nitroanilide was monitored at 412 nm

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Then the aged mice were intraperitoneally injected with FOXO4-DRI (5 mg/kg) in PBS or PBS alone every other day for three administrations

Pham-HuyFree radicals, antioxidants in disease and healthInt J Biomed Sci: IJBS, 4 (2008), p