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Precision-cut intestinal slices as in vitro tool for studies on drug metabolism

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For giredestrant treatments, 8.05 10 7 cells were passaged on treatment day 3 into fresh 0.6 nM giredestrant, compound and medium were replenished every 34 days, and one set of cells for each replicate was collected on treatment days 10 and 17 (7.00 10 7 to 8.00 10 7 cells per sample)