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Taken together, we have identified four drugs that show an additive/synergistic inhibitory effect in the proliferation assay and as monotherapy in the viability screen in all four tested melanoma cell lines

This includes 1,569 genes stimulated by 5099% and 583 genes suppressed by 5099%

amino acids oxidases, cyclooxygenase, lipoxygenase, nitric oxide synthase, xanthine oxidase, NADPH oxidase), oxidative protein folding in the endoplasmic reticulum (ER), the oxidation of free fatty acids (FFAs) in peroxisomes and microsomes, through the effect of pro-inflammatory cytokines, and in the mitochondrial respiratory chain

The cells were grown to sub-confluence, harvested with trypsin (#35553-74, Nacalai Tesque, Kyoto, Japan), and suspended in phosphate-buffered saline (PBS, #14249-95, Nacalai Tesque, Kyoto, Japan)