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Thus, considering the limitations encountered with animal models, like a low grade in mimicking the dose relevant sensitivities (132134), the proposed OoC system offered many advantages compared to classic in vitro or rodent models

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L.et al (2023c)

In both types of cells, upon ER stress induction by thapsigargin (Tg) treatment, all the three UPR pathways were activated, as determined by phosphorylation of IRE1, PERK, eIF2, and cleavage of ATF6 to generate the activated form, as well as increased expression of the downstream transcription factors XBP1s and ATF4 (Fig