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BPC-157 operates upstream and at the cell surface - modulating growth-factor receptor signaling, transcription factor expression, and vascular tone - while TB-500 operates inside the cell, regulating the cytoskeletal substrate that allows the cells responding to those signals to actually migrate into the repair zone

To understand the underlying mechanisms, inhibitors targeting the Notch pathway (DAPT) and NF-B pathway (BMS) were also applied to HUVEC and murine CLI models

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