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Antibody-stained protein signal was amplified and visualized using SuperSignal West Pico PLUS chemiluminescent substrate (Thermo Fisher #34578) and imaged with a ChemiDoc MP Imaging system (Bio-Rad)

Cellular viability assays Cells were seeded in 384-well plates at an approximate number of 6001000 cells per well

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This makes the injection ideal for individuals with chronic tiredness, high stress, or demanding work schedules

Studies including females are lacking, which is a significant gap since sex differences with respect to the endocannabinoid system are well-described (Laurikainen et al., 2019