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Breifly, 15ul supernatant was isocratically eluted through a 4.6 150 mm C18 column (ESA, Inc Chelmsford, MA) with a mobile phase containing 50mM LiH 2 PO 4 , 1.0mM 1-octanesulfonic acid and 1.5% (v/v) methanol and detected by a 2-channel Coulochem III electrochemical detector (ESA, Inc