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Subsequently, 0.1 mM IPTG was added to the transfected cells, which was then cultured with 1 L LB medium for 16 h at 27 C

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A gene encoding a mitogen activated protein kinase kinase kinase in induced simultaneously with genes for a mitogen-activated protein kinase and S6 ribosomal protein kinase by touch, cold, and water stress in Arabidopsis thaliana