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For each sample, 1 g of RNA was retrotranscribed into cDNA using SuperScript III Reverse Transcriptase (Thermo Fisher Scientific, catalog no.18080093) as indicated by the provider

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The lower taxa meant lower prestige and were therefore often relegated to the domain of women as the exploration of fungi and other lower taxa (such as lichens, algae, and mosses) was not considered a serious concern to the male institutions of higher learning