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According to the Tobacco 21: Model Policy backed by a consortium of national public health groups, strong MLSA policies: Define tobacco products to include current and future tobacco products, including e-cigarettes Prohibit the sale of tobacco products to persons under the age of 21 Require the tobacco retailer or their employee to verify the age of the purchaser prior to the sale Require tobacco retailers to post signs stating that sales to persons under the age of 21 are prohibited Designate an enforcement agency and establish a clear enforcement protocol Create a tobacco retail licensing program if the jurisdiction has the authority to do so under state law Dedicate funding to fully cover enforcement costs, either through licensing fees or as a provision in a state statute or local ordinance Provide authority for the state, county, or municipality to inspect tobacco retailers for compliance with MLSA 21 and a mandated minimum number of annual compliance checks for every tobacco retail establishment Provide penalties focused on the tobacco retailer or licensee rather than the youth purchaser or non-management employee

Overall, the results are largely consistent with our iTRAQ-based proteomics quantification results ( Figure 5 )

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