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Targeting the Achilles heel of multidrug-resistant cancer by exploiting the fitness cost of resistance

Defining the optimal selenium dose for prostate cancer risk reduction: insights from the U-shaped relationship between selenium status, DNA damage, and apoptosis

METHOD DETAILS Sample preparation FOXO4 FHD (95-195) was subcloned into the pET His6 Tobacco Etch Virus (TEV) LIC cloning vector (2B-T, obtained from Scott Gradia, Addgene Plasmid #29666) and transformed into BL21 (DE3) cells

The metabolism of glutamate and glutamine in the body is quite complicated because glutamine is produced from glutamate in muscles

It involves a series of tests and experiments to ensure that the method meets certain criteria and that the results are valid, reproducible and meaningful