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[DOI] [PMC free article] [PubMed] [Google Scholar] 81.McCarty MF, Thomas CA

821 These and other findings reveal that Zn II coordination by the CxCC motif slightly destabilizes the G-domain, while subsequent addition of either substrate analog GTPS and product GDP induce significant stabilization of a metastable or fluxional G-domain (Figure 47)

gently swirl or roll between palms to aid dissolution Allow the vial to stand for 510 minutes at room temperature to ensure complete dissolution Inspect the solution for clarity, particulates, or colour changes Concentration Calculation 10 mg peptide in 1 mL = 10 mg/mL 10 mg peptide in 2 mL = 5 mg/mL Choose concentration based on assay requirements and dosing calculations Important Notes Never use tap water, distilled water, or non-sterile water for reconstitution Do not shake vigorously (may cause foam formation and peptide denaturation) If the peptide does not dissolve completely, gentle warming to 37 C (not exceeding) may aid dissolution Do not freeze-thaw reconstituted solutions repeatedly